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Exchange 2010 Edge Transport with TMG and Exchange 2010/2013 on premises.

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So I have a really wierd issue. Any help would be greatly appreciated.

Internally we have a 2010/2013 org with:

Exchange 2010 - 2x CAS&HT , 2xMBX, 2xEdge Exchange 2013 - 2x CAS, 2xMBX

I am trying to subscribe my Edge Transport servers, that also run TMG firewall, to the Exchange org after installing Exchange 2013, so that I can send email out through Exchange 2013. However, I cannot telnet on port 25 from one of my 2013 Mailbox Servers to the Edge Transport. This works:

2013MX1 to EDGETMG02 2013MX2 to EDGETMG01 2013MX2 to EDGETMG02

However, if I telnet on port 25 from 2013MX1 to EDGETMG01 then I get an error 421 4.3.2 Service Not Available. What does this mean, and what can I do to fix this? At the moment email just queues up on Exchange 2013 without any route out to the internet.

Edit:

Exchange 2013 servers are all SP1 CU6 Exchange 2010 servers are all SP3 RU4


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